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论文题目 Identification of Cold-Responsive miRNAs and Their Target Genes in Nitrogen-Fixing Nodules of Soybean
作  者 Senlei Zhang (张森磊), Youning Wang (王幼宁), Kexue Li(李科学), Yanmin Zou (邹艳敏), Liang Chen(陈亮) , and Xia Li(李霞)*;
发表年度 2014
刊物名称 International Journal of Molecular Sciences
卷、期、页码 ; 15, 13596-13614;
影响因子
论文摘要

  As a warm climate species, soybean is highly sensitive to chilling temperatures. Exposure to chilling temperatures causes a significant reduction in the nitrogen fixation rate in soybean plants and subsequent yield loss. However, the molecular basis for the sensitivity of soybean to chilling is poorly understood. In this study, we identified cold-responsive miRNAs in nitrogen-fixing nodules of soybean. Upon chilling, the expression of gma-miR397a, gma-miR166u and gma-miR171p was greatly upregulated, whereas the expression of gma-miR169c, gma-miR159b, gma-miR319a/b and gma-miR5559 was significantly decreased. The target genes of these miRNAs were predicted and validated using 5' complementary DNA ends (5'-RACE) experiments, and qPCR analysis identified putative genes targeted by the cold-responsive miRNAs in response to chilling temperatures. Taken together, our results reveal that miRNAs may be involved in the protective mechanism against chilling injury in mature nodules of soybean. 

全文链接 http://dx.doi.org/10.3390/ijms150813596
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